Model : HD-4FL/4FL+ et HD-8FL/8FL+
The Halo Counter is an automated fluorescence cell counter designed for research laboratories, cell analysis platforms, and hospital environments requiring fast, reproducible, and reliable results.
It enables automated cell counting, fluorescence-based viability analysis (AO/PI, GFP, RFP), and precise identification of live and dead cells, powered by advanced artificial intelligence algorithms.
Operating without proprietary consumables, with a wide dynamic range and multi-sample capacity, the Halo Counter optimizes cell culture workflows while reducing operating costs.
- Up to 2 to 4 samples analyzed simultaneously
- No proprietary consumables required
- Reduced handling time
- Ideal for high-throughput environments
👉 Need to validate the configuration best suited to your cells and analysis throughput?
Contact an Izibio expert for personalized support.
Why choose a Halo Counter fluorescence cell counter?
This fluorescence cell counter stands out through a unique combination of analytical performance, ease of use, and operational flexibility.
It has been developed to meet the needs of laboratories where precision, repeatability, and high throughput are essential.
Count multiple samples simultaneously without consumables
The Halo Counter enables parallel analysis of multiple samples without proprietary consumables, reducing costs and simplifying protocols.
The analysis is fully automated thanks to high-resolution imaging and integrated algorithms, ensuring reliable and reproducible results from one sample to another.
This functionality is particularly well suited for high-throughput laboratories (platforms, core facilities, academic and hospital laboratories), where efficiency and reproducibility are critical.
Variable magnification according to cell size
This optical adaptability makes the Halo Counter ideal for analyzing a wide range of samples, from mammalian cells to microorganisms, using a single instrument.
The Halo Counter offers 4×, 8×, and 10× objectives, allowing the analysis to be precisely adapted to the size and morphology of the cells studied.
This flexibility ensures reliable detection for both mammalian cells and smaller cells, while maintaining accurate and reproducible segmentation.
Choosing the appropriate magnification improves result quality and supports the standardization of cellular analyses.
Advanced artificial intelligence algorithms
The Halo Counter integrates artificial intelligence algorithms specifically trained for cell analysis in fluorescence and bright-field.
They enable:
- reliable cell segmentation
- reduced errors related to debris
- superior reproducibility compared with manual methods
- accurate analysis even at high cell density
In practice, Halo Counter AI results in reduced analysis time, improved segmentation accuracy, and standardized results, regardless of the operator.
Intuitive user interface and simplified workflow
The Halo Counter interface is designed for immediate usability, even by non-expert users.
Acquisition settings, result analysis, and data export are accessible in just a few clicks, reducing the risk of operator error. This ergonomic design enables seamless integration of the fluorescence cell counter into daily laboratory workflows.
This user-friendly approach facilitates routine use of the Halo Counter, even by non-specialists, reducing errors and accelerating analyses.
Specifications
The HD-4FL / HD-4FL+ and HD-8FL / HD-8FL+ models cover configurations suitable for both research laboratories and high-throughput platforms.
The choice mainly depends on the number of samples to be processed, fluorescence requirements, and analysis throughput.
| Caractéristique | HD-4FL/4FL+ | HD-8FL/8FL+ |
|---|---|---|
| Modèle | HD-4FL/4FL+ | HD-8FL/8FL+ |
| Chambres d'échantillons | 4 | 8 |
| Objectif | 4x ou 4 & 10x | 4x ou 4 & 10x |
| Plage de taille cellulaire | 0.8~400µm | 0.8~400µm |
| Canal optique | Brightfield, Trypan blue,AO/PI, GFP, RFP | Brightfield, Trypan blue,AO/PI, GFP, RFP |
| Plage de concentration | 1×10³ ~ 3×10⁷ cellules/ml | 1×10³ ~ 3×10⁷ cellules/ml |
| Vues de capture | 1, 3 (normal), 4, 5 | 1, 3 (normal), 4, 5 |
| Volume d'échantillon | 5 µl (50 µm), 20 µl (200 µm), 40 µl (400 µm) | 5 µl (50 µm), 20 µl (200 µm), 40 µl (400 µm) |
| Champ clair | LED blanche | LED blanche |
| Lumière d’excitation | 488 nm, 587 nm LED | 488 nm, 587 nm LED |
| Filtres d’émission | 535 nm et 600 LP | 535 nm et 600 LP |
| Mise au point | Auto focus | Auto focus |
| Caméra | 6.3 MP CMOS | 6.3 MP CMOS |
Performance, compatibility, and laboratory compliance
The Halo Counter combines advanced analytical performance, broad compatibility with routinely used culture supports, and compliance suited to laboratory requirements, including regulated environments (e.g., GMP), to ensure reliable and well-documented results.
FAQ – Halo Counter fluorescence cell counter (Izibio)
It is an instrument that automates cell counting and, in addition to bright-field imaging, enables the analysis of fluorescent signals (e.g. viability, markers), providing faster, more reproducible results that are less operator-dependent.
Yes. The Halo Counter is designed to operate without proprietary consumables, reducing recurring costs and simplifying laboratory logistics.
The Halo Counter supports bright-field imaging and fluorescence channels including AO/PI, GFP, and RFP.
The Halo Counter covers a wide range of cell sizes, from 0.8 to 400 µm, thanks to 4×, 8×, and 10× objectives, depending on the model.
Yes. Thanks to its size range and available objectives, the Halo Counter can be adapted to different sample types, including yeast and certain microorganisms, depending on the protocol and configuration.
Yes. The Halo Counter can be integrated into regulated (GMP) environments depending on the selected configuration. IQ/OQ documentation and expert support can be provided to validate compliance with laboratory requirements.
Yes. Depending on the model, the Halo Counter enables multi-sample analysis using multiple chambers (e.g. 4- or 8-chamber configurations).
The Halo Counter accepts different sample volumes, typically 5 µL, 20 µL, or 40 µL, depending on the chamber height and format used.
The Halo Counter is compatible with commonly used laboratory supports, including T25 flasks, culture dishes, 6-well plates, and hemocytometers.
You can contact an Izibio application expert to validate suitability for your applications (cell types, fluorescence, throughput), request a quote, or schedule a demonstration.
Results (cell concentration, viability, counts, images depending on configuration) can be exported for analysis, archiving, and batch tracking, enabling seamless integration into laboratory workflows.
